Digging for biosynthetic dark matter.

An analysis of bacterial communities in soil samples from around the world reveals unexplored diversity in biosynthetic enzymes

Sosa, Customary International Law, and the Continuing Relevance of Erie

Ten years ago, the conventional wisdom among international law academics was that customary international law (CIL) had the status of self-executing federal common law to be applied by courts without any need for political branch authorization. This modern position came under attack by so-called revisionist critics who argued that CIL had the status of federal common law only in the relatively rare situations in which the Constitution or political branches authorized courts to treat it as such. Modern position proponents are now claiming that the Supreme Court\u27s 2004 decision in Sosa v. Alvarez-Machain confirms that CIL has the status of self-executing federal common law. As this Article explains, the decision in Sosa did not in fact embrace the modern position, and, indeed, is best read as rejecting it. Commentators who construe Sosa as embracing the modern position have confounded the automatic incorporation of CIL as domestic federal law in the absence of political branch authorization (i.e., the modern position) with the entirely different issue of whether and to what extent a particular statute, the Alien Tort Statute ( ATS ), authorizes courts to apply CIL as domestic federal law. The Article also explains how CIL continues to be relevant to domestic federal common law despite Sosa\u27s rejection of the modern position. The fundamental flaw of the modern position is that it ignores the justifications for, and limitations on, post-Erie federal common law. As the Article shows, however, there are a number of contexts in addition to the ATS in which it is appropriate for courts to develop federal common law by reference to CIL, including certain jurisdictional contexts not amenable to state regulation (namely admiralty and interstate disputes), and gap-filling and interpretation of foreign affairs statutes and treaties. The Article concludes by considering several areas of likely debate during the next decade concerning the domestic status of CIL: corporate aiding and abetting liability under the ATS; application of CIL to the war on terrorism; and the use of foreign and international materials in constitutional interpretation

Pollen‐ And Seed‐Sterility In Hybrids

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141411/1/ajb205743.pd

In-Betweening Disney: An Animated History of Hollywood Labor and Ideological Imagineering, 1935-1947

The Walt Disney Company’s meticulously-crafted corporate mythos, as it developed in the mid-twentieth century, hid a conflicted history of anti-New Deal, nationalist ideology that was popularized during the clashes of the Hollywood labor movement in the 1930s and 1940s. In 1935, the National Labor Relations Act was passed as Disney’s Snow White and the Seven Dwarfs (1937) entered full-scale production, and each were central to the labor-management conflict that lurked behind the scenes of the motion picture industry. By the mid-1940s, following the conclusion of the Second World War, Congress passed the Labor Management Relations (Taft-Hartley) Act and imposed a series of restrictions on U.S. labor organizations, including those in Hollywood. Compounding matters further, Hollywood’s elite and the federal government alleged that the specter of Communism had infiltrated major motion picture studios. In 1947, Walt Disney, among several others in the film industry, placed their fears on record before the House Un-American Activities Committee. The biographies of men like Walt Disney have continued to dominate historical discussions related to U.S. industry and cultural production respectively. To date, however, there has been a peculiar disinterest in the artists and laborers who toiled behind the scenes of the U.S. film making industry. This inattention to those artists has created noticeable gaps in the historiography of U.S. labor, more generally. In an effort to elevate the voices of Hollywood’s working class, this project places various primary sources, including organizational records, artwork, and oral history interviews in conversation with corporate and government sources. This thesis deconstructs the myth making carried out by the Walt Disney Company, and places the animation studio’s history at the intersection of U.S. labor organization and the proliferation of anti-New Deal ideology. This study argues that Walt Disney’s status as successful Hollywood industrialist, asset of the federal government, and rabid anti-New Dealer fueled his campaign to demonize the organizational efforts of his artists and depict their assertions of federally-guaranteed labor rights as the subversive actions of cartoonish villains

English King and German Commoner: An Exploration of Sixteenth Century Clothing and Identity

This paper will explore the connections of clothing and identity in the sixteenth century. The fit and construction of clothing can be directly related to how a person is perceived, or indeed, how one perceives one\u27s self. Henry VIII (1491-1547) of England will be compared and contrasted with Matthäus Schwarz (1496-1574), a commoner from Augsburg, Germany. Tudor will represent how identity can be created for others, particularly through legislation and courtly life; while Schwarz\u27 own words will assist in the exploration of the identity of the individual

A National Portrait of Domestic Violence Courts

A growing number of criminal courts nationwide handle domestic violence cases on separate calendars, termed domestic violence courts. There are now 208 confirmed domestic violence courts across the U.S. (Center for Court Innovation 2009). More than 150 similar projects have been established internationally. Some domestic violence courts emerged in the context of the broader "problem-solving court" movement and share characteristics with other specialized courts, such as separate dockets and specially trained judges. However, the origins of domestic violence courts are also distinct, growing out of the increased attention afforded domestic violence matters by the justice system over the past 30 years. With funding from the National Institute of Justice, this study explores how criminal domestic violence courts have evolved, their rationale, and how their operations vary across the U.S. This study does not test whether domestic violence courts reduce recidivism, protect victims, or achieve other specific effects -- although we provide a thorough literature review on these points. Rather, our aim is to present a comprehensive national portrait of the field as it exists today, laying the groundwork for future information exchange and research

Vibration limiting of rotors by feedback control

Experimental findings of a three mass rotor with four channels of feedback control are reported. The channels are independently controllable with force being proportional to the velocity and/or instantaneous displacement from equilibrium of the shaft at the noncontacting probe locations (arranged in the vertical and horizontal attitudes near the support bearings). The findings suggest that automatic feedback control of rotors is feasible for limiting certain vibration levels. Control of one end of a rotor does afford some predictable vibration limiting of the rotor at the other end

Hydrothermal modeling for optimum temperature control : an estimation-theoretic approach

Originally presented as part of the first author's thesis, (Environmental Engineer) in the M.I.T. Dept. of Civil EngineeringA short-term temperature forecasting (STF) system is proposed to predict and control plant intake and discharge temperatures at Salem Harbor Electric Generating Station. It is desired to minimize receiving-water (i.e., intake-water) temperatures during peak power demand periods, in order to minimize the cost of complying with the maximum discharge water temperature limit. This study addresses the hydrothermal modeling requirements of an STF system. An important element of an STF system is a predictive model of plant intake water temperatures. For application to Salem Harbor Station, strict model performance criteria exist, defining a model development problem: Develop a simple model to predict plant intake water temperatures 24 hours ahead, predicting daily peak intake temperatures within 10F on 90% of the days, and using only existing measurements. An estimation-theoretic approach to model development is used, which quantifies and minimizes the uncertainties in the model. The approach employs optimal filtering and full-information maximum- likelihood (FIML) estimation to obtain optimum parameter estimates. A two-basin, two-layer hydrothermal model of Salem Harbor is developed. The model computes hourly intake temperatures, incorporating tidal flushing, stratification, surface heat exchange, and wind advection of the plume. Twenty-eight model parameters and five noise statistics are estimated from intake-temperature data. Preliminary best-fit parameter values are obtained subjectively, followed by FIML parameter estimation using a data base of 96 hourly measurements (7/29 - 8/2/74). The model is tested for 106 days (5/17- 9/20/74) and various performance measures are computed, including sum- of-squares of measurement residuals (S), whiteness (P), percent of daily peak temperature predictions within 10F of actual (T), and others. Visual inspection of 24-hour intake temperature predictions shows that the two-basin, two-layer model performs qualitatively well. However, the model fails statistical tests on S and P, indicating structural weaknesses. FIML estimation yields physically unrealistic values for certain parameters, probably compensating for inadequate model structure. Despite structural flaws in the two-basin, two-layer model, FIML estimation yields parameters with consistently better performance than the preliminary estimates (by a small amount). It is concluded that the two-basin, two-layer model is presently unsuitable for STF use, largely due to structural weaknesses. Possible corrections are suggested; however, a statistical model of hourly temperatures appears to offer greater potential accuracy than physically-derived models. FIML parameter estimation is shown to be useful for water quality model development on a real system, particularly after subjective model development has been exhausted.New England Power Company under the MIT Energy Laboratory Electric Power Progra

Pass-back chain extension expands multimodular assembly line biosynthesis.

Modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymatic assembly lines are large and dynamic protein machines that generally effect a linear sequence of catalytic cycles. Here, we report the heterologous reconstitution and comprehensive characterization of two hybrid NRPS-PKS assembly lines that defy many standard rules of assembly line biosynthesis to generate a large combinatorial library of cyclic lipodepsipeptide protease inhibitors called thalassospiramides. We generate a series of precise domain-inactivating mutations in thalassospiramide assembly lines, and present evidence for an unprecedented biosynthetic model that invokes intermodule substrate activation and tailoring, module skipping and pass-back chain extension, whereby the ability to pass the growing chain back to a preceding module is flexible and substrate driven. Expanding bidirectional intermodule domain interactions could represent a viable mechanism for generating chemical diversity without increasing the size of biosynthetic assembly lines and challenges our understanding of the potential elasticity of multimodular megaenzymes

Predicting polaron mobility in organic semiconductors with the Feynman variational approach

We extend the Feynman variational approach to the polaron problem \cite{Feynman1955} to the Holstein (lattice) polaron. This new theory shows a discrete transition to small-polarons is observed in the Holstein model. The method can directly used in the FHIP \cite{Feynman1962} mobility theory to calculate dc mobility and complex impedance. We show that we can take matrix elements from electronic structure calculations on real materials, by modelling charge-carrier mobility in crystalline rubrene. Good agreement is found to measurement, in particular the continuous thermal transition in mobility from band-like to thermally-activated, with a minimum in mobility predicted at 140 K.Comment: 8 pages, 6 figure